Always secure the rotor lid tightly onto the rotor before closing the main centrifuge housing lid. Programming the Run Set the speed using the SPEED arrows. Set the duration using the TIME arrows. Set the target cooling parameters using the TEMP arrows. Press START . 4. Cleaning, Preventive Maintenance, and Care
Adjust the temperature, time, and speed dials to your desired experimental protocol.
Electronic lid release button. It only operates when the rotor is completely stationary and the unit is powered on.
If spinning an odd number of samples, prepare a "balance tube" containing water to equal the weight of the sample tube.
Disclaimer: As the 5402 is an older model, ensure you are utilizing the latest manufacturer-issued documentation for safety. eppendorf 5402 manual updated
Always load tubes symmetrically. For example, if spinning 2 tubes, place them directly opposite each other (180 degrees apart).
The updated manual emphasizes the kinetic energy limits of the rotor system. The 5402 is designed to balance speed (RPM) with Relative Centrifugal Force (RCF) efficiently.
Ultimate Guide to the Eppendorf 5402 Centrifuge: Operations, Maintenance, and Updated Manual Resources
0 to 99 minutes or a "momentary" button for short pulses. Essential Operating Instructions 1. Pre-Cooling (Fast Cool) Always secure the rotor lid tightly onto the
Shows the set speed or current running speed.
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120V / 230V options (dependent on regional model variants), 50–60 Hz 2. Interface and Control Panel Overview
Turn on the main power switch located on the side or rear of the unit. Set the target cooling parameters using the TEMP arrows
If using 0.2 mL PCR tubes or 0.5 mL microcentrifuge tubes, ensure the appropriate Eppendorf adapters are placed in opposite slots symmetrically.
As physical copies of older manuals get lost or damaged, maintaining a digital archive is critical for laboratory compliance and auditing. Where to Look
: Typically reaches up to 14,000 RPM (approx. 16,000 x g), depending on the rotor. Cooling System
Open the lid. Inspect the rotor chamber for debris, moisture, or signs of corrosion. Ensure the rotor is securely fastened to the motor shaft using the provided rotor key. Loading and Balancing the Rotor
If you encounter Error 6, it may indicate a loose rotor, converter overload, or a defective brake. Wait 5 minutes after switching the unit off before restarting.